Oncostatin M antibody

Catalog No. ABIN116203

This Rabbit Polyclonal antibody specifically detects Oncostatin M in WB, IHC (p), EIA and Func. It exhibits reactivity toward Human.

Quick Overview for Oncostatin M antibody (ABIN116203)

Target: Oncostatin M (OSM)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Oncostatin M antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-Oncostatin M Antibody

Specificity: Human Oncostatin M.

Purification: Affinity Chromatography employing immobilized Human Oncostatin M matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant Human Oncostatin M.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhuman Oncostatin M (10 ng/mL), a concentration of 0.1 - 0.175 μg/mL of this antibody isrequired. Immunohistochemistry: We recommend use of 1 g per ml as a starting dilution. Paraffinsections may require pretreatment like Proteinase K or Trypsin predigestion to improvestaining. ELISA: To detect human Oncostatin M by direct ELISA (using 100 l/well antibody solution)a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant human Oncostatin M. Western Blot: use a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatiblesecondary reagents the detection limit for recombinant human Oncostatin M is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for Oncostatin M

Target: Oncostatin M (OSM)

Alternative Name: Oncostatin-M

Background: Synonyms: OSM

Molecular Weight: approx. 150 KDa

Gene ID: 5008

NCBI Accession: NP_065391

UniProt: P13725

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion