PDGFA antibody

Catalog No. ABIN116208

The Rabbit Polyclonal anti-PDGFA antibody (ABIN116208) specifically detects PDGFA in WB, IHC (p), EIA and Func. The antibody is reactive with Human samples.

Quick Overview for PDGFA antibody (ABIN116208)

Target: PDGFA (Platelet Derived Growth Factor A (PDGFA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDGFA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-PDGFA Antibody

Specificity: Human Platelet Derived Growth Factor AA (hPDGF-AA).

Purification: Affinity Chromatography using an immobilized Human PDGF-AA matrix.

Immunogen: Highly pure (>98%) E.coli derived recombinant Human PDGF-AA (Cat.-No PA118).

Application Details

Application Notes: Neutralisation: To yield one-half maximal inhibition [ND50] of the biological activity ofhPDGF-AA (3.0 ng/mL), a concentration of 0.03 - 0.05 μg/mL of this antibody is required. Immunohistochemistry: A starting dilution of 1 μg/mL is recommended for frozen sections. ELISA: To detect hPDGF-AA by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5 g/ ml of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant hPDGF-AA. Western Blot: To detect hPDGF-AA by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.5 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hPDGF-AA is 1.5 -3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for PDGFA

Target: PDGFA (Platelet Derived Growth Factor A (PDGFA))

Alternative Name: PDGFA

Background: PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF AA, PDGF AB and PDGF BB. The three isoforms bind with different affinities to two receptor types, A and B, which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers, the A subunit of PDGF binds only to A receptors with high affinity, whereas the B subunit can bind to both A and B receptors. Evidence suggests that PDGF may function as a neurotrophic factor. The fact that PDGF-A receptors are expressed in oligodendrocyte progenitor cells, whereas PDGF-B receptors are expressed on neurons, suggests that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system by paracrine and autocrine routes.Synonyms: PDGF A, PDGF alpha, PDGF subunit A, PDGF-1, PDGF-A, PDGF1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha, Platelet-derived growth factor subunit A

Gene ID: 5154

NCBI Accession: NP_002598

UniProt: P04085

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling