VEGFA antibody

Catalog No. ABIN116252

The Rabbit Polyclonal anti-VEGFA antibody is suitable to detect VEGFA in samples from Human. It has been validated for WB, EIA and Func.

Quick Overview for VEGFA antibody (ABIN116252)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VEGFA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-VEGFA Antibody

Specificity: This antibody detects Vascular endothelial growth factor A.

Purification: Affinity chromatography employing immobilized hVEGF matrix

Immunogen: Highly pure (>98%) recombinant hVEGF (human VEGF)

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhVEGF (10 ng/mL), a concentration of 0.05 - 0.1 μg/mL of this antibody is required. ELISA: Indirect: To detect hVEGF by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hVEGF. Sandwich: To detect hVEGF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction Biotinylated Anti-Human VEGF as a detection antibody, allows thedetection of at least 0.2 - 0.4 ng/well of recombinant hVEGF. Western Blot: To detect hVEGF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: Lyophilized from PBS, pH 7.2, None

Handling Advice: This product is photosensitive and should be protected from light. Should it contain a precipitate we recommend microcentrifugation before use.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Alternative Name: VEGF-A

Background: Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor

Gene ID: 7422

NCBI Accession: NP_001020537

UniProt: P15692

Pathways: RTK Signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling