CCL15 antibody (Biotin)

Catalog No. ABIN116283

The Biotin-conjugated Goat Polyclonal anti-CCL15 antibody (ABIN116283) specifically detects CCL15 in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for CCL15 antibody (Biotin) (ABIN116283)

Target: CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL15 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CCL15 Antibody (Biotin)

Specificity: This antibody reacts with Macrophage Inflammatory Protein-5.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Affinity chromatography

Immunogen: Highly pure (>98%) recombinant hMIP-5 (human MIP-5)

Application Details

Application Notes: ELISA: To detect hMIP-5-alpha by direct ELISA (using 100 μL/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthMIP-5-alpha. Western blot: To detect hMIP-5-alpha by Western Blot analysis this antibody can be used ata concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hMIP-5-alpha is 1.5 - 3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CCL15

Target: CCL15 (Chemokine (C-C Motif) Ligand 15 (CCL15))

Alternative Name: MIP5 / CCL15

Background: Macrophage inflammatory protein 5 (CCL15) is a member of the human beta-chemokine family and is chemotactic for T cells and monocytes but not neutrophils, eosinophils, or B cells. It induces changes in intracellular calcium concentration in monocytes and is thought to act through the CCR1 receptor but also binds to CCR3.Synonyms: C-C motif chemokine 15, Chemokine CC-2, Leukotactin-1, MIP-1 delta, MIP-5, Macrophage inflammatory protein 5, Mrp-2b, NCC-3, NCC3, SCYA15, Small-inducible cytokine A15

Gene ID: 6359

NCBI Accession: NP_116741

UniProt: Q16663