IL12A antibody

Catalog No. ABIN116323

This Goat Polyclonal antibody specifically detects IL12A in WB, EIA and Func. It exhibits reactivity toward Mouse.

Quick Overview for IL12A antibody (ABIN116323)

Target: IL12A (Interleukin 12 alpha (IL12A))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IL12A antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-IL12A Antibody

Specificity: Interleukin-12 (IL-12)

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant mouse IL-12

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofmIL-12 (0.3 ng/mL), a concentration of 0.012 - 0.2 μg/mL of this antibody is required. ELISA: To detect mIL-12 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant mIL-12. Western Blot: To detect mIL-12 alpha by Western Blot analysis this antibody can be used ata concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mIL-12 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Immunohistochemistry: We recommend use of 1 g per ml as a starting dilution. Paraffinsections may require pretreatment like Proteinase K or Trypsin predigestion to improvestaining.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IL12A

Target: IL12A (Interleukin 12 alpha (IL12A))

Alternative Name: Interleukin-12 alpha / IL12A

Background: Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, NK cell stimulatory factor chain 1, NKSF1

Gene ID: 16159

NCBI Accession: NP_032377

UniProt: P43431

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation