CXCL13 antibody (Biotin)

Catalog No. ABIN116341

This Rabbit Polyclonal antibody specifically detects CXCL13 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for CXCL13 antibody (Biotin) (ABIN116341)

Target: CXCL13 (Chemokine (C-X-C Motif) Ligand 13 (CXCL13))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL13 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CXCL13 Antibody (Biotin)

Specificity: This antibody detects Human CXCL13 / BCA1.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogen: Highly pure (>98%) E.coli derived recombinant Human CXCL13 / BCA1

Application Details

Application Notes: ELISA: To detect hBCA-1 by direct ELISA (using 100 ml/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hBCA-1. Western Blot: To detect hBCA-1 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 mg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hBCA-1 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.

Target Details for CXCL13

Target: CXCL13 (Chemokine (C-X-C Motif) Ligand 13 (CXCL13))

Alternative Name: CXCL13 / BCA1

Background: B lymphocyte chemoattractant, independently cloned and named Angie, is a CXC chemokine strongly expressed in the follicles of the spleen, lymph nodes, and Peyer's patches. It preferentially promotes the migration of B lymphocytes (compared to T cells and macrophages), apparently by stimulating calcium influx into, and chemotaxis of, cells expressing Burkitt's lymphoma receptor 1 (BLR1). It may therefore function in the homing of B lymphocytes to follicles.Synonyms: B cell-attracting chemokine 1, B lymphocyte chemoattractant, BLC, C-X-C motif chemokine 13, CXC chemokine BLC, SCYB13, Small-inducible cytokine B13

Molecular Weight: 150 kDa

Gene ID: 10563

NCBI Accession: NP_006410

UniProt: O43927