IL-3 beta antibody (Biotin)

Catalog No. ABIN116367

The Biotin-conjugated Goat Polyclonal anti-IL-3 beta antibody (ABIN116367) specifically detects IL-3 beta in WB and EIA. The antibody is reactive with Rat samples.

Quick Overview for IL-3 beta antibody (Biotin) (ABIN116367)

Target: IL-3 beta (Interleukin 3 beta (IL-3 beta))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This IL-3 beta antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IL-3 beta Antibody (Biotin)

Specificity: Rat Interleukin-3 beta

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant rIL-3 beta.

Application Details

Application Notes: ELISA: To detect rat IL-3 beta by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinantrat IL-3 beta. Western Blot: To detect rat IL-3 beta by Western Blot analysis this antibody can be used ata concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant rat IL-3 beta is 1.5 - 3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for IL-3 beta

Target: IL-3 beta (Interleukin 3 beta (IL-3 beta))

Alternative Name: Interleukin-3 beta / IL3 beta

Background: IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells.Synonyms: IL-3 beta, IL3B

Gene ID: 24495

NCBI Accession: NP_113701

UniProt: P97688