IL-3 beta antibody

Catalog No. ABIN116369

The Goat Polyclonal anti-IL-3 beta antibody is suitable to detect IL-3 beta in samples from Rat. It has been validated for WB, EIA and Func.

Quick Overview for IL-3 beta antibody (ABIN116369)

Target: IL-3 beta (Interleukin 3 beta (IL-3 beta))

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This IL-3 beta antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Product Details anti-IL-3 beta Antibody

Specificity: Rat Interleukin-3 beta

Cross-Reactivity (Details): Species reactivity (tested):Rat.

Purification: Affinity chromatography

Immunogen: Highly pure (>98%) recombinant rIL-3 beta.

Application Details

Application Notes: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of RatIL-3beta (100 ng/mL), a concentration of 2.7-4.0 μg/mL of this antibody is required. ELISA: To detect rat IL-3 beta by direct ELISA (using 100 μL/well antibody solution) thisantibody can be used at a concentration of 0.15-0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinantrat IL-3 beta. Western Blot: To detect rat IL-3 beta by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant rat IL-3 beta is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for one month or at -20 °C for longer.

Target Details for IL-3 beta

Target: IL-3 beta (Interleukin 3 beta (IL-3 beta))

Alternative Name: Interleukin-3 beta / IL3 beta

Background: IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells.Synonyms: IL-3 beta, IL3B

Gene ID: 24495

NCBI Accession: NP_113701

UniProt: P97688