CD80 antibody (Biotin)

Catalog No. ABIN116382

This anti-CD80 antibody is a Rabbit Polyclonal antibody detecting CD80 in EIA. Suitable for Mouse.

Quick Overview for CD80 antibody (Biotin) (ABIN116382)

Target: CD80

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD80 antibody is conjugated to Biotin

Application: Enzyme Immunoassay (EIA)

Product Details anti-CD80 Antibody (Biotin)

Specificity: This antibody reatcts with Murine B-71

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant mB-71.

Application Details

Application Notes: ELISA: To detect mB-71 by direct ELISA (using 100 l/well antibody solution) this antibody can beused at a concentration of 0.15-0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant mB-71. Western Blot: To detect mB-71 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mB-71 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore to a concentration of 50 μg/mL with sterile PBS solution containing 0.1 % BSA.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for CD80

Target: CD80

Alternative Name: CD80

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80

Gene ID: 12519

NCBI Accession: NP_033985

UniProt: Q00609

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints