Midkine antibody (Biotin)

Catalog No. ABIN116440

This Rabbit Polyclonal antibody specifically detects Midkine in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for Midkine antibody (Biotin) (ABIN116440)

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Midkine antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Midkine Antibody (Biotin)

Purification: Affinity chromatography.

Immunogen: Highly pure (>98%) recombinant hMidkine.

Application Details

Application Notes: ELISA: To detect hMidkine by direct ELISA (using 100 l/well antibody solution) thisantibody can be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction withcompatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinanthMidkine. Western Blot: To detect hMidkine by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hMidkine is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore to a concentration of 50 μg/mL with sterile PBS solution containing 0.1 % BSA.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for Midkine

Target: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Alternative Name: Midkine

Background: Midkine exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Its expression is predominant only for a short period from approximately one-half to two-thirds of the way through gestation, before and after that, it is barely detectable. Midkine was first found in differentiating mouse teratocarcinoma cells. It has neurotrophic activities and is mitogenic to certain, but not to all, fibroblast cell lines.Synonyms: Amphiregulin-associated protein, MDK, MK, MK1, Midgestation and kidney protein, NEGF2, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein

Gene ID: 4192

NCBI Accession: NP_001012333

UniProt: P21741

Pathways: RTK Signaling, M Phase, Skeletal Muscle Fiber Development