Resistin antibody

Catalog No. ABIN116449

This Goat Polyclonal antibody specifically detects Resistin in WB and EIA. It exhibits reactivity toward Mouse.

Quick Overview for Resistin antibody (ABIN116449)

Target: Resistin (RETN)

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This Resistin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Resistin Antibody

Purification: Affinity chromatography employing immobilized mResistin matrix.

Immunogen: Highly pure (>98%) recombinant mResistin.

Application Details

Application Notes: Neutralization. ELISA: To detect mResistin by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant mResistin. Western Blot: To detect mResistin by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant mResistin is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of > 0.2 mg/mL.

Buffer: 0.5X PBS pH 7.4 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Target Details for Resistin

Target: Resistin (RETN)

Alternative Name: Resistin

Background: Synonyms: ADSF, Adipose tissue-specific secretory factor, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, Cysteine-rich secreted protein FIZZ3, FIZZ3, HXCP1, RETN, RSTN

Gene ID: 57264

NCBI Accession: NP_001191888

UniProt: Q99P87

Pathways: Feeding Behaviour, Smooth Muscle Cell Migration