GDF3 antibody (Biotin)

Catalog No. ABIN116495

The Rabbit Polyclonal anti-GDF3 antibody is suitable to detect GDF3 in samples from Human. It has been validated for WB and EIA.

Quick Overview for GDF3 antibody (Biotin) (ABIN116495)

Target: GDF3 (Growth Differentiation Factor 3 (GDF3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GDF3 antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-GDF3 Antibody (Biotin)

Purification: Affinity chromatography.

Immunogen: Highly pure (> 98%) recombinant hGDF-3 (human GDF-3).

Application Details

Application Notes: ELISA: To detect hGDF-3 by direct ELISA (using 100 l/well antibody solution) this antibodycan be used at a concentration of 0.15 - 0.30 μg/mL. Used in conjunction with compatiblesecondary reagents, allows the detection of at least 0.2 ng/well of recombinant hGDF-3. Western blot: To detect hGDF-3 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGDF-3 is 1.5 -3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore to a concentration of 50 μg/mL with sterile PBS solution containing 0.1 % BSA.

Buffer: BPS without preservatives.

Preservative: Without preservative

Storage: 4 °C

Storage Comment: Store the antibody at 2-8 °C for one year from despatch.

Expiry Date: 12 months

Target Details for GDF3

Target: GDF3 (Growth Differentiation Factor 3 (GDF3))

Alternative Name: GDF3

Background: GDF3 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. The function of this protein is unknown, but expression studies suggest it may be involved in regulation of the adult lymphatic and erythroid systems and embryonic development.Synonyms: GDF-3, Growth/differentiation factor 3

Gene ID: 9573

NCBI Accession: NP_065685

UniProt: Q9NR23