APOC3 antibody

Catalog No. ABIN116546

The Goat Polyclonal anti-APOC3 antibody is suitable to detect APOC3 in samples from Human. It has been validated for WB, IP, IHC (p), IHC (fro) and EIA.

Quick Overview for APOC3 antibody (ABIN116546)

Target: APOC3 (Apolipoprotein C-III (APOC3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-APOC3 Antibody

Specificity: This product has been prepared by Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1 % cross reactivity against other types of ApoLipoprotein was detected by ELISA against purified standards. This antibody recognizes Human ApoLipoprotein C-III and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, E and J ApoLipoproteins. Non-specific cross reaction of anti-ApoLipoprotein antibodies with other Human serum proteins is negligible.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic full-length Apolipoprotein Type C-III (not selected epitopes) using conventional peptide technology.

Application Details

Application Notes: This Apolipoprotein antibody has been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation, Western blotting forhighly sensitive qualitative analysis and for Immunohistochemistry. Recommended Dilutions: ELISA: 1/5,000-1/10,000. Western blot: 1/5,000-1/10,000. Immunoprecipitation: 1/100. Immunohistochemistry Paraffin Sections: 2.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, with 0.01 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use.

Storage: -20 °C

Storage Comment: Store the antibody undiluted (in aliquots) at -20 °C.

Target Details for APOC3

Target: APOC3 (Apolipoprotein C-III (APOC3))

Alternative Name: Apolipoprotein C III (Apo CIII)

Background: At least 9 distinct polymorphic forms of apolipoproteins are known. The apolipoproteins act as stabilizers of the intact lipoprotein particles. Quantitative measurements of HDL, LDL and VLDL particles in human serum are often used to estimate an individuals' relative risk of coronary heart disease. In addition, quantitative immunological measurements of certain apolipoproteins (especially A1 and B) have been suggested to be more accurate estimators of coronary heart disease than measurements of lipoprotein particles (especially HDL and LDL). Apolipoprotein C-III is a very low density lipoprotein (VLDL) protein. It inhibits lipoprotein lipase and hepatic lipase and it is thought to delay catabolism of triglyceride-rich particles. An increase in ap °C-III levels induces the development of hypertriglyceridemia.Synonyms: Apo-CIII, Ap °C-III, Ap °C3, Apolipoprotein C-III, Apolipoprotein C3

Gene ID: 345

NCBI Accession: NP_000031

UniProt: P02656

Pathways: Carbohydrate Homeostasis, Lipid Metabolism