PKM2 antibody

Catalog No. ABIN116809

This Goat Polyclonal antibody specifically detects PKM2 in WB, IP and EIA. It exhibits reactivity toward Rabbit.

Quick Overview for PKM2 antibody (ABIN116809)

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This PKM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-PKM2 Antibody

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Pyruvate Kinase [Rabbit Muscle]. Cross reactivity may occur against other mammalian Pyruvate Kinase, but were not specifically determined.

Purification: Prepared from monospecific antiserum by delipidation and defibrination.

Immunogen: Pyruvate Kinase from Rabbit Muscle.

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation. Recommended Dilutions: This product has been assayed against 1.0 g of Pyruvate Kinase[Rabbit Muscle] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Goat IgG and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as asubstrate for 30 minutes at room temperature. A working dilution of 1: 2,000 to 1: 12,000 ofthe reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 40.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Alternative Name: PKM2

Background: Synonyms: CTHBP, Cytosolic thyroid hormone-binding protein, M2-PK, OIP-3, OIP3, Opa-interacting protein 3, PK2, PK3, PKM, Pyruvate kinase 2/3, Pyruvate kinase M1/M2, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle, Pyruvate kinase muscle isozyme, THBP1

Gene ID: 100008676

NCBI Accession: NP_001182573

UniProt: P11974

Pathways: Warburg Effect