Urate Oxidase antibody

Catalog No. ABIN116849

The Goat Polyclonal anti-Urate Oxidase antibody is suitable to detect Urate Oxidase in samples from Bacillus. It has been validated for WB, IP and EIA.

Quick Overview for Urate Oxidase antibody (ABIN116849)

Target: Urate Oxidase (UOX)

Reactivity: Bacillus

Host: Goat

Clonality: Polyclonal

Conjugate: This Urate Oxidase antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-Urate Oxidase Antibody

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Uricase [Bacillus species].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Diutions: This product has been assayed against 1.0 μg of Uricase [Bacillusspecies] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 4,000 to1: 36,000 of the reconstitution concentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for Urate Oxidase

Target: Urate Oxidase (UOX)

Alternative Name: Uricase

Background: Synonyms: EC 1.7.3.3, UOX, URIC, URO, Urate oxidase

Gene ID: 36824

UniProt: Q45697