Acetylcholinesterase antibody

Catalog No. ABIN116884

This Goat Polyclonal antibody specifically detects Acetylcholinesterase in WB, IP and EIA. It exhibits reactivity toward Cow.

Quick Overview for Acetylcholinesterase antibody (ABIN116884)

Target: Acetylcholinesterase (AChE)

Reactivity: Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This Acetylcholinesterase antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Product Details anti-Acetylcholinesterase Antibody

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Acetyl Cholinesterase [Bovine Erythrocytes]. Cross reactivity against Acetyl Cholinesterase from other sources is unknown.

Purification: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Acetyl Cholinesterase [Bovine Erythrocytes].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Diutions: This product has been assayed against 1.0 g of AcetylCholinesterase [Bovine Erythrocytes] in a standard sandwich ELISA using Peroxidaseconjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 20,000 to 1: 100,000 of the reconstitution concentration issuggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for Acetylcholinesterase

Target: Acetylcholinesterase (AChE)

Alternative Name: Acetylcholinesterase

Background: Synonyms: AChE, Acetylcholine Esterase, Acetylcholine-Esterase

Gene ID: 540446

NCBI Accession: NP_001069688

UniProt: P23795

Pathways: Skeletal Muscle Fiber Development