NOTCH2 antibody

Catalog No. ABIN1169006

There are 2+ publications for this product available. The Rat Monoclonal anti-NOTCH2 antibody is suitable to detect NOTCH2 in samples from Human and Mouse. It has been validated for FACS.

Quick Overview for NOTCH2 antibody (ABIN1169006)

Target: NOTCH2 (Notch 2 (NOTCH2))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This NOTCH2 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 16F11

Product Details anti-NOTCH2 Antibody

Purpose: anti-Notch2, mAb (16F11)

Specificity: Recognizes human and mouse endogenous Notch2 receptor.

Characteristics: Monoclonal Antibody. Recognizes human and mouse endogenous Notch-2 receptor. Isotype: Rat IgG1kappa. Clone: 16F11. Applications: FACS. Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide. Notch signaling pathway regulates many different cell fate decisions in both vertebrate and invertebrate species. There are 5 canonical Notch ligands in mammals: Jagged-1, Jagged-2, DLL1, DLL3 and DLL4. These can bind to the four Notch receptors Notch 1-4. It is important for pattern formation during development such as neurogenesis, angiogenesis or myogenesis and regulates T cell development and stem cell maintenance. Notch signaling is also involved in cellular processes through-out adulthood. Signaling via Notch occurs between neighbouring cells and both the receptor and its ligands are transmembrane proteins.

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant mouse Notch2:Fc (AG-40B-0110).

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-NOTCH2 antibody (ABIN1169006)

Ribeiro, Hasan, Wilson, Boucontet, Pereira, Lesjean-Pottier, Satoh-Takayama, Di Santo, Vosshenrich: "Cutting edge: Thymic NK cells develop independently from T cell precursors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 9, pp. 4993-7, (2010) (PubMed).

Fiorini, Merck, Wilson, Ferrero, Jiang, Koch, Auderset, Laurenti, Tacchini-Cottier, Pierres, Radtke, Luther, Macdonald: "Dynamic regulation of notch 1 and notch 2 surface expression during T cell development and activation revealed by novel monoclonal antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 11, pp. 7212-22, (2009) (PubMed).

Target Details for NOTCH2

Target: NOTCH2 (Notch 2 (NOTCH2))

Alternative Name: Notch2

Background:

Alternate Names/Synonyms: Neurogenic Locus Notch Homolog Protein 2, Motch B

Product Description: Notch signaling pathway regulates many different cell fate decisions in both vertebrate and invertebrate species. There are 5 canonical Notch ligands in mammals: Jagged-1, Jagged-2, DLL1, DLL3 and DLL4. These can bind to the four Notch receptors Notch 1-4. It is important for pattern formation during development such as neurogenesis, angiogenesis or myogenesis and regulates T cell development and stem cell maintenance. Notch signaling is also involved in cellular processes through-out adulthood. Signaling via Notch occurs between neighbouring cells and both the receptor and its ligands are transmembrane proteins.

UniProt: O35516

Pathways: Notch Signaling, Stem Cell Maintenance