Recombinant Myosin 9 antibody

Catalog No. ABIN1169007

The Human Monoclonal anti-Myosin 9 antibody (Clone SF9) (ABIN1169007) specifically detects Myosin 9 in WB, ELISA and ICC. The antibody is reactive with Human, Mouse, Rat and Drosophila melanogaster samples.

Quick Overview for Recombinant Myosin 9 antibody (ABIN1169007)

Target: Myosin 9 (MYH9)

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Drosophila melanogaster

Host: Human

Clonality: Monoclonal

Conjugate: This Myosin 9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Clone: SF9

Product Details anti-Myosin 9 Antibody

Purpose: anti-Myosin IIA (non-muscle) (heavy chain), mAb (rec.) (SF9)

Specificity: Recognizes human, mouse, rat and drosophila myosin IIA (heavy chain).

Characteristics: Recombinant Antibody. Recognizes human, mouse, rat and drosophila myosin IIA (heavy chain). Species cross-reactivity: Drosophila, Human, Mouse, Rat. Clone: SF9. Isotype: Human IgG2lambda. Applications: ELISA, ICC, WB. Host: Purified from HEK 293 cell culture supernatant. Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide. anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Purification: Purified from HEK 293 cell culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Full length myosin IIA from rat liver.

Isotype: IgG2 lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

-20°C

Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details for Myosin 9

Target: Myosin 9 (MYH9)

Alternative Name: Myosin IIA

Background:

Alternate Names/Synonyms: Cellular Myosin Heavy Chain, Type A, Myosin Heavy Chain 9, Myosin Heavy Chain, Non-muscle IIa, Non-muscle Myosin Heavy Chain A

Product Description: anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

UniProt: P35579

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Integrin Complex