LAG3 antibody

Catalog No. ABIN1169112

Cited in 4+ publications. The Mouse Monoclonal anti-LAG3 antibody (Clone 1-10E-04) (ABIN1169112) specifically detects LAG3 in WB, ELISA, IHC, ICC, IP and Func. The antibody is reactive with Human and Monkey samples.

Quick Overview for LAG3 antibody (ABIN1169112)

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAG3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Functional Studies (Func)

Clone: 1-10E-04

Product Details anti-LAG3 Antibody

Specificity: Recognizes human and monkey LAG-3.

Cross-Reactivity: Human, Monkey

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.001EU/μg purified protein or <1EU/mg purified protein (LAL test, Lonza).

Immunogen: Recombinant human LAG-3.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-LAG3 antibody (ABIN1169112)

Ward, Hall, Cairns, Leggat, Urbaniak, Vickers, Barker: "Clonal regulatory T cells specific for a red blood cell autoantigen in human autoimmune hemolytic anemia." in: Blood, Vol. 111, Issue 2, pp. 680-7, (2008) (PubMed).

Triebel, Hacene, Pichon: "A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors." in: Cancer letters, Vol. 235, Issue 1, pp. 147-53, (2006) (PubMed).

Huard, Mastrangeli, Prigent, Bruniquel, Donini, El-Tayar, Maigret, Dréano, Triebel: "Characterization of the major histocompatibility complex class II binding site on LAG-3 protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 11, pp. 5744-9, (1997) (PubMed).

Huard, Gaulard, Faure, Hercend, Triebel: "Cellular expression and tissue distribution of the human LAG-3-encoded protein, an MHC class II ligand." in: Immunogenetics, Vol. 39, Issue 3, pp. 213-7, (1994) (PubMed).

Target Details for LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Alternative Name: LAG-3

Background: Lymphocyte activation gene 3 (LAG-3, CD223) plays an important role in negatively regulating T cell proliferation, function and homeostasis. It is required for maximal natural and induced regulatory T cell (Treg) function. LAG-3 is closely related to the T cell co-receptor CD4 and binds to MHC class II molecules but with a significantly higher affinity than CD4.

UniProt: P18627

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints