IL1A antibody

Catalog No. ABIN1169128

This Mouse Monoclonal antibody specifically detects IL1A in ELISA. It exhibits reactivity toward Mouse.

Quick Overview for IL1A antibody (ABIN1169128)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL1A antibody is un-conjugated

Application: ELISA

Clone: Bamboo-2

Product Details anti-IL1A Antibody

Purpose: anti-IL-1alpha (mouse), mAb (Bamboo-2)

Specificity: Recognizes mouse IL-1alpha.

Characteristics: Monoclonal Antibody. Recognizes mouse IL-1alpha. Isotype: Mouse IgG1kappa. Clone: Bamboo-2. Applications: ELISA. Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide. The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1alpha and IL-1beta. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1alpha was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1alpha upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1alpha is not mediated by caspase-1 and is not required for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1alpha and IL-1beta. Although most activators fully rely on the inflammasome for IL-1alpha secretion, some induce the processing and secretion of IL-1alpha in an inflammasome-independent manner.

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant mouse mature IL-1alpha.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

-20°C

Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL-1alpha

Background:

Alternate Names/Synonyms: Interleukin-1alpha IL-1alpha

Product Description: The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1alpha and IL-1beta. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1alpha was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1alpha upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1alpha is not mediated by caspase-1 and is not required for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1alpha and IL-1beta. Although most activators fully rely on the inflammasome for IL-1alpha secretion, some induce the processing and secretion of IL-1alpha in an inflammasome-independent manner.

UniProt: P01582

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints