HCV 1b NS5B antibody

Catalog No. ABIN1169141

Product Details anti-HCV 1b NS5B Antibody

Target: HCV 1b NS5B (HCV NS5b) (Hepatitis C Virus NS5b (HCV NS5b))

Reactivity: Hepatitis C Virus (HCV)

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCV 1b NS5B antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Recognizes HCV NS5B (epitope mapped to NS5B aa 372-382). It recognizes genotype 1 a and also 1b (albeit with lower affinity).

Cross-Reactivity: Hepatitis C Virus (HCV)

Cross-Reactivity (Details): It does not recognize genotype 2a.

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase (genotype 1a).

Clone: 5B-3B1

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-HCV 1b NS5B antibody (ABIN1169141)

Kapadia, Chisari: "Hepatitis C virus RNA replication is regulated by host geranylgeranylation and fatty acids." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 7, pp. 2561-6, (2005) (PubMed).

Quinkert, Bartenschlager, Lohmann: "Quantitative analysis of the hepatitis C virus replication complex." in: Journal of virology, Vol. 79, Issue 21, pp. 13594-605, (2005) (PubMed).

Svitkin, Pause, Lopez-Lastra, Perreault, Sonenberg: "Complete translation of the hepatitis C virus genome in vitro: membranes play a critical role in the maturation of all virus proteins except for NS3." in: Journal of virology, Vol. 79, Issue 11, pp. 6868-81, (2005) (PubMed).

Hardy, Marcotrigiano, Blight, Majors, Rice: "Hepatitis C virus RNA synthesis in a cell-free system isolated from replicon-containing hepatoma cells." in: Journal of virology, Vol. 77, Issue 3, pp. 2029-37, (2003) (PubMed).

Randall, Grakoui, Rice: "Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 1, pp. 235-40, (2003) (PubMed).

Moradpour, Bieck, Hügle, Wels, Wu, Hong, Blum, Bartenschlager: "Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 593-601, (2002) (PubMed).

Target Details for HCV 1b NS5B

Target: HCV 1b NS5B (HCV NS5b) (Hepatitis C Virus NS5b (HCV NS5b))

Abstract: HCV NS5b Products

Target Type: Viral Protein

Background: The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.