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Hepatitis C Virus NS5b (HCV NS5b) (AA 111-130) antibody Primary Antibody

HCV NS5b Reactivity: Hepatitis C Virus (HCV) IF, ELISA, WB Host: CHO Cells Monoclonal 9A2
Catalog No. ABIN956403
$558.28
Plus shipping costs $45.00
100 μg
local_shipping Shipping to: United States
Delivery in 8 to 11 Business Days
  • Target
    Binding Specificity
    AA 111-130
    Reactivity
    Hepatitis C Virus (HCV)
    Host
    CHO Cells
    Clonality
    Monoclonal
    Conjugate
    Un-conjugated
    Application
    Immunofluorescence (IF), ELISA, Western Blotting (WB)
    Sequence
    LSSKAVNHIR SVWKDLLEDT
    Specificity
    Human, HCV subtype 1b NS5B
    Characteristics
    Partially humanized, chimeric recombinant monoclonal
    Purification
    purifiedby protein G affinity chromatography
    Immunogen
    Recombinant Hepatitis C Virus subtype 1b nonstructuralprotein 5B (NS5B) RNA-dependent RNA polymerase (RdRp)
    Clone
    9A2
    Isotype
    IgG1
  • Application Notes
    ELISAtiter 1:1000, Western immunoblotting, reduced conditions, dilution 1:1000, Immunofluorescense 1:1000. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/ml
    Buffer
    PBS pH 7.4, with 0.1%
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Divide antibodyinto aliquots prior usage. Avoid multiple freeze-thaw cycles as productdegradation may result.
    Storage
    -20 °C
    Storage Comment
    This product isshipped in non-frozen liquid form in ambient conditions
  • Target
    Background
    Non-structural protein 5B (NS5B)represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, whichis a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronichepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunitRNA-dependent RNA polymerase is absolutely essential for the viral replication.Antibodyis derived from mouse anti-HCV NS5B monoclonal antoibody 9A2 expressing mousehybridoma culture. cDNA of antibody variable regions were isolated and clonedinto the human IgG1 constant region-containing antibody expression vector.Partially humanized, chimeric antibody was expressed by QMCF CHOEBNALT85 cellline. Antibody is used for high-specific efficient detection of soluble ormembrane-bound proteins fused with 9A2 epitope-tag sequence. For moreinformation please visit: http://icosagen.com/cell-based-assay/qmcf-assay-overview
    UniProt
    Q9WMX2
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