HCV 1b NS5B antibody (AA 111-130)

Catalog No. ABIN956403

The CHO Cells Monoclonal anti-HCV 1b NS5B antibody (Clone 9A2) (ABIN956403) specifically detects HCV 1b NS5B in WB, ELISA and IF. The antibody is reactive with Hepatitis C Virus (HCV) samples.

Quick Overview for HCV 1b NS5B antibody (AA 111-130) (ABIN956403)

Target: HCV 1b NS5B (HCV NS5b) (Hepatitis C Virus NS5b (HCV NS5b))

Reactivity: Hepatitis C Virus (HCV)

Host: CHO Cells

Clonality: Monoclonal

Conjugate: This HCV 1b NS5B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 9A2

Product Details anti-HCV 1b NS5B Antibody

Binding Specificity: AA 111-130

Sequence: LSSKAVNHIR SVWKDLLEDT

Specificity: Human, HCV subtype 1b NS5B

Characteristics: Partially humanized, chimeric recombinant monoclonal

Purification: purifiedby protein G affinity chromatography

Immunogen: Recombinant Hepatitis C Virus subtype 1b nonstructuralprotein 5B (NS5B) RNA-dependent RNA polymerase (RdRp)

Isotype: IgG1

Application Details

Application Notes: ELISAtiter 1:1000, Western immunoblotting, reduced conditions, dilution 1:1000, Immunofluorescense 1:1000. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/ml

Buffer: PBS pH 7.4, with 0.1%

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Divide antibodyinto aliquots prior usage. Avoid multiple freeze-thaw cycles as productdegradation may result.

Storage: -20 °C

Storage Comment: This product isshipped in non-frozen liquid form in ambient conditions

Target Details for HCV 1b NS5B

Target: HCV 1b NS5B (HCV NS5b) (Hepatitis C Virus NS5b (HCV NS5b))

Target Type: Viral Protein

Background: Non-structural protein 5B (NS5B)represents the RNA-dependent RNA polymerase (RdRp) of Hepatitis C Virus, whichis a small positive strand RNA virus in the family Flaviviridae. HCV is a major causative agent of acute and chronichepatitis, hepatocellular carcinoma and liver cirrhosis. The single subunitRNA-dependent RNA polymerase is absolutely essential for the viral replication.Antibodyis derived from mouse anti-HCV NS5B monoclonal antoibody 9A2 expressing mousehybridoma culture. cDNA of antibody variable regions were isolated and clonedinto the human IgG1 constant region-containing antibody expression vector.Partially humanized, chimeric antibody was expressed by QMCF CHOEBNALT85 cellline. Antibody is used for high-specific efficient detection of soluble ormembrane-bound proteins fused with 9A2 epitope-tag sequence. For moreinformation please visit: http://icosagen.com/cell-based-assay/qmcf-assay-overview

UniProt: Q9WMX2