Recombinant TUBB antibody

Catalog No. ABIN1169288

The Human Monoclonal anti-TUBB antibody has been validated for WB, ELISA and ICC. It is suitable to detect TUBB in samples from Human, Mouse, Rat, Pig, Monkey and Drosophila melanogaster.

Quick Overview for Recombinant TUBB antibody (ABIN1169288)

Target: TUBB (Tubulin, beta (TUBB))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Pig, Monkey, Drosophila melanogaster

Host: Human

Clonality: Monoclonal

Conjugate: This TUBB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Clone: S11B

Product Details anti-TUBB Antibody

Specificity: Recognizes human, mouse, rat, pig, drosophila and monkey beta-tubulin.

Cross-Reactivity: Fruit Fly (Drosophila melanogaster), Human, Monkey, Mouse (Murine), Pig (Porcine), Rat (Rattus)

Purification: Purified from HEK 293 cell culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Full length tubulin from pig brain.

Isotype: IgG2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

anti-beta-Tubulin, monoclonal antibody (recombinant) (S11B) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: beta-Tubulin

Background: Anti-beta-Tubulin, monoclonal antibody (recombinant)(S11B) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Pathways: Microtubule Dynamics, M Phase