Recombinant TUBB antibody

Catalog No. ABIN1169288

The Human Monoclonal anti-TUBB antibody is suitable to detect TUBB in samples from Human, Mouse, Rat, Monkey, Pig and Drosophila melanogaster. It has been validated for WB, ICC and ELISA.

Quick Overview for Recombinant TUBB antibody (ABIN1169288)

Target: TUBB (Tubulin, beta (TUBB))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse, Rat, Monkey, Pig, Drosophila melanogaster

Host: Human

Clonality: Monoclonal

Conjugate: This TUBB antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), ELISA

Clone: S11B

Product Details anti-TUBB Antibody

Purpose: anti-beta-Tubulin, mAb (rec.) (S11B)

Specificity: Recognizes human, mouse, rat, pig, drosophila and monkey beta-tubulin.

Characteristics: Recombinant Antibody. Recognizes human, mouse, rat, pig, drosophila and monkey beta-tubulin. Species cross-reactivity: Drosophila, Human, Monkey, Mouse, Pig, Rat. Clone: S11B. Isotype: Human IgG2lambda. Applications: ELISA, ICC, WB. Host: Purified from HEK 293 cell culture supernatant. Liquid. In PBS containing 10 % glycerol and 0.02 % sodium azide. anti-beta-Tubulin, monoclonal antibody (recombinant)(S11B) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Purification: Purified from HEK 293 cell culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Full length tubulin from pig brain.

Isotype: IgG2 lambda

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

anti-beta-Tubulin, monoclonal antibody (recombinant) (S11B) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: After opening, prepare aliquots and store at -20 °C.Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment:

+4°C

-20°C

Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: beta-Tubulin

Background:

Alternate Names/Synonyms: Tubulin beta Chain, Tubulin beta-5 chain

Product Description: anti-beta-Tubulin, monoclonal antibody (recombinant)(S11B) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

Pathways: Microtubule Dynamics, M Phase