TRAIL antibody

Catalog No. ABIN1169389

This Mouse Monoclonal antibody specifically detects TRAIL in WB. It exhibits reactivity toward Human. It has been mentioned in 4+ publications

Quick Overview for TRAIL antibody (ABIN1169389)

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRAIL antibody is un-conjugated

Application: Western Blotting (WB)

Clone: HS501

Product Details anti-TRAIL Antibody

Specificity: Recognizes human TRAIL.

Cross-Reactivity: Human

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant human TRAIL.

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-TRAIL antibody (ABIN1169389)

Luetzkendorf, Mueller, Mueller, Caysa, Nerger, Schmoll: "Growth inhibition of colorectal carcinoma by lentiviral TRAIL-transgenic human mesenchymal stem cells requires their substantial intratumoral presence." in: Journal of cellular and molecular medicine, Vol. 14, Issue 9, pp. 2292-304, (2010) (PubMed).

Wenger, Mattern, Penzel, Gassler, Haas, Sprick, Walczak, Krammer, Debatin, Herr: "Specific resistance upon lentiviral TRAIL transfer by intracellular retention of TRAIL receptors." in: Cell death and differentiation, Vol. 13, Issue 10, pp. 1740-51, (2006) (PubMed).

Huber, Fais, Iero, Lugini, Canese, Squarcina, Zaccheddu, Colone, Arancia, Gentile, Seregni, Valenti, Ballabio, Belli, Leo, Parmiani, Rivoltini: "Human colorectal cancer cells induce T-cell death through release of proapoptotic microvesicles: role in immune escape." in: Gastroenterology, Vol. 128, Issue 7, pp. 1796-804, (2005) (PubMed).

Washburn, Weigand, Grosse-Wilde, Janke, Stahl, Rieser, Sprick, Schirrmacher, Walczak: "TNF-related apoptosis-inducing ligand mediates tumoricidal activity of human monocytes stimulated by Newcastle disease virus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 4, pp. 1814-21, (2003) (PubMed).

Target Details for TRAIL

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Alternative Name: TRAIL

Background: TNF-related apoptosis-inducing ligand (TRAIL, Apo2L,CD253, TNFSF10) is a type II transmembrane protein of about 34 kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-R1 (DR4, Apo2, CD261, TNFRSF10A), TRAIL-R2 (DR5, KILLER, TRICK2A, TRICK2B, CD262, TNFRSF10B), TRAIL-R3 (DcR1, LIT, TRID, CD263, TNFRSF10C), TRAIL-R4 (DcR2, TRUNDD, CD264, TNFRSF10D), and osteoprotegerin (OPG, OCIF, TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC). .

UniProt: P50591

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity