CDC25B antibody (AA 377-566)

Catalog No. ABIN1171796

The Rabbit Polyclonal anti-CDC25B antibody has been validated for WB, IHC, ICC and IP. It is suitable to detect CDC25B in samples from Human.

Quick Overview for CDC25B antibody (AA 377-566) (ABIN1171796)

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-CDC25B Antibody

Binding Specificity: AA 377-566

Purpose: Polyclonal Antibody to Cell Division Cycle Protein 25B (CDC25B)

Specificity: The antibody is a rabbit polyclonal antibody raised against CDC25B. It has been selected for its ability to recognize CDC25B in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPQ124Hu01Recombinant Cell Division Cycle Protein 25B (CDC25B)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CDC25B

Target: CDC25B (Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B))

Alternative Name: CDC25B

Background: CDC25HU2, M-phase inducer phosphatase 2, Dual specificity phosphatase Cdc25B

Pathways: Cell Division Cycle, M Phase, Autophagy