Angiopoietin 1 antibody (AA 284-452) (Biotin)
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- Target See all Angiopoietin 1 (ANGPT1) Antibodies
- Angiopoietin 1 (ANGPT1)
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Binding Specificity
- AA 284-452
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Angiopoietin 1 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA
- Sequence
- MGHHHHHHSG SEF-RDCADVY QAGFNKSGIY TIYINNMPEP KKVFCNMDVN GGGWTVIQHR EDGSLDFQRG WKEYKMGFGN PSGEYWLGNE FIFAITSQRQ YMLRIELMDW EGNRAYSQYD RFHIGNEKQN YRLYLKGHTG TAGKQSSLIL HGADFSTKDA DNDNCMCKCA LMLTGGWWFD AC
- Specificity
- It has been selected for its ability to recognize ANGPT1 in immunohistochemical staining and Western blotting.
- Purification
- Affinity Chromatography
- Immunogen
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Recombinant ANGPT1 expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against ANGPT1 conjugated to biotin. - Isotype
- IgG
- Top Product
- Discover our top product ANGPT1 Primary Antibody
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- Application Notes
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Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Comment
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Content: The quality control contains recombinant ANGPT1 (Arg284~Cys452) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % . - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze/thaw cycles
- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Expiry Date
- 12 months
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- Target
- Angiopoietin 1 (ANGPT1)
- Abstract
- ANGPT1 Products
- Synonyms
- AGP1 antibody, AGPT antibody, ANG1 antibody, 1110046O21Rik antibody, Ang-1 antibody, Ang1 antibody, Agpt antibody, Agpt1 antibody, ang1 antibody, wu:fc76g06 antibody, ANG-1 antibody, angpt1 antibody, MGC80788 antibody, angiopoietin 1 antibody, angiopoietin 1 S homeolog antibody, ANGPT1 antibody, Angpt1 antibody, angpt1 antibody, angpt1.S antibody
- Pathways
- RTK Signaling, Glycosaminoglycan Metabolic Process
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