PML antibody (AA 59-239)

Catalog No. ABIN1175605

This Rabbit Polyclonal antibody specifically detects PML in WB. It exhibits reactivity toward Human.

Quick Overview for PML antibody (AA 59-239) (ABIN1175605)

Target: PML (Promyelocytic Leukemia (PML))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PML antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PML Antibody

Binding Specificity: AA 59-239

Purpose: Polyclonal Antibody to Promyelocytic Leukemia Protein (PML)

Specificity: The antibody is a rabbit polyclonal antibody raised against PML. It has been selected for its ability to recognize PML in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: RPC221Hu01Recombinant Promyelocytic Leukemia Protein (PML)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PML

Target: PML (Promyelocytic Leukemia (PML))

Alternative Name: Promyelocytic Leukemia Protein

Background: MYL, RNF71, TRIM19, Probable Transcription Factor PML, Ring Finger Protein 71, Tripartite motif-containing protein 19

Pathways: p53 Signaling, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus