NGFR antibody

Catalog No. ABIN1176837

This anti-NGFR antibody is a Mouse Monoclonal antibody detecting NGFR in WB, FACS, IHC (p), IP, ICC, ICFC and MS. Suitable for Human, Non-Human Primate, Rabbit, Cat and Ferret. This Primary Antibody has been cited in 4+ publications.

Quick Overview for NGFR antibody (ABIN1176837)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivity: Human, Non-Human Primate, Rabbit, Cat, Ferret

Host: Mouse

Clonality: Monoclonal

Conjugate: This NGFR antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Intracellular Flow Cytometry (ICFC), Mass Spectrometry (MS)

Clone: NGFR5

Product Details anti-NGFR Antibody

Purpose: Anti-Hu CD271 Purified

Specificity: The mouse monoclonal antibody NGFR5 (originally C34C) recognizes an epitope within ammino acids 1 - 160 of CD271/NGFR, a 75 kDa transmembrane glycoprotein of the TNFR superfamily.

No Cross-Reactivity: Mouse, Rat

Cross-Reactivity (Details): Human, Non-Human Primates, Feline (Cat), Rabbit, Ferret

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Purified CD271 protein isolated from human melanoma cell line A875

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Recommended dilution: 1-2 μg/mL, Positive tissue: melanoma, heat-mediated antigen retrieval.
Flow cytometry: Extracellular and intracellular staining, recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-NGFR antibody (ABIN1176837)

Alpers, Hudkins, Ferguson, Johnson, Schatteman, Bothwell: "Nerve growth factor receptor expression in fetal, mature, and diseased human kidneys." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 69, Issue 6, pp. 703-13, (1994) (PubMed).

Thompson, Schatteman, Gown, Bothwell: "A monoclonal antibody against nerve growth factor receptor. Immunohistochemical analysis of normal and neoplastic human tissue." in: American journal of clinical pathology, Vol. 92, Issue 4, pp. 415-23, (1989) (PubMed).

Schatteman, Gibbs, Lanahan, Claude, Bothwell: "Expression of NGF receptor in the developing and adult primate central nervous system." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 8, Issue 3, pp. 860-73, (1988) (PubMed).

Marano, Dietzschold, Earley, Schatteman, Thompson, Grob, Ross, Bothwell, Atkinson, Koprowski: "Purification and amino terminal sequencing of human melanoma nerve growth factor receptor." in: Journal of neurochemistry, Vol. 48, Issue 1, pp. 225-32, (1987) (PubMed).

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: CD271

Background: Nerve growth factor receptor,CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells.,TNFRSF16, NGFR, p75NTR, p75NGFR

Gene ID: 4804

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding