ITGA4 antibody

Catalog No. ABIN1176852

This anti-ITGA4 antibody is a Rat Monoclonal antibody detecting ITGA4 in FACS, IHC and BR. Suitable for Mouse. This Primary Antibody has been cited in 5+ publications.

Quick Overview for ITGA4 antibody (ABIN1176852)

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Blocking Reagent (BR)

Clone: DATK32

Product Details anti-ITGA4 Antibody

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: AKR/Cum mouse T lymphoma line TK1

Isotype: IgG2a kappa

Application Details

Application Notes: Flow cytometry: Because expression of LPAM-1 on peripheral lymphocytes is at very low density, we recommend amplification of the signal through the use of a biotinylated second-step antibody plus a bright third-step reagent, such as Streptavidin-PE (Cat. no. 554061), for detection of the antigen by flow cytometry. Other reported applications include in vitro blocking of adhesion, induction of cellular aggregation, and in vivo blocking of lymphocyte homing to mucosal sites.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-ITGA4 antibody (ABIN1176852)

Andrew, Rott, Kilshaw, Butcher: "Distribution of alpha 4 beta 7 and alpha E beta 7 integrins on thymocytes, intestinal epithelial lymphocytes and peripheral lymphocytes." in: European journal of immunology, Vol. 26, Issue 4, pp. 897-905, (1996) (PubMed).

Andrew, Berlin, Honda, Yoshino, Hamann, Holzmann, Kilshaw, Butcher: "Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 9, pp. 3847-61, (1994) (PubMed).

Hamann, Andrew, Jablonski-Westrich, Holzmann, Butcher: "Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3282-93, (1994) (PubMed).

Berlin, Berg, Briskin, Andrew, Kilshaw, Holzmann, Weissman, Hamann, Butcher: "Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1." in: Cell, Vol. 74, Issue 1, pp. 185-95, (1993) (PubMed).

Kilshaw, Murant: "Expression and regulation of beta 7(beta p) integrins on mouse lymphocytes: relevance to the mucosal immune system." in: European journal of immunology, Vol. 21, Issue 10, pp. 2591-7, (1991) (PubMed).

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: LPAM-1

Background: The DATK32 antibody reacts with an epitope specific to the alpha4beta7 integrin heterodimer. alpha4beta7 (LPAM-1) is expressed on most mature lymphocytes and on small subsets of thymic and bone marrow cells. It interacts with several ligands, including VCAM-1 (CD106), fibronectin, and MAdCAM-1. DATK32 antibody induces alpha4beta7-dependent lymphocyte aggregation, but it inhibits other alpha4beta7- mediated lymphocyte adhesion events, including binding to fibronectin, MAdCAM-1, and VCAM-1 (CD106).
Synonyms: Integrin alpha4beta7 complex

Pathways: Integrin Complex