CD45 antibody

Catalog No. ABIN1176960

Cited in 17+ publications. The Rat Monoclonal anti-CD45 antibody (Clone RA3-6B2) (ABIN1176960) specifically detects CD45 in FACS, IP, IHC (p), IHC (fro), IHC (zinc), BR and IHC (f). The antibody is reactive with Human and Mouse samples.

Quick Overview for CD45 antibody (ABIN1176960)

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD45 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: RA3-6B2

Product Details anti-CD45 Antibody

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse Abelson Leukemia Virus-Induced pre-B tumor cells

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD45 antibody (ABIN1176960)

Bleesing, Morrow, Uzel, Fleisher: "Human T cell activation induces the expression of a novel CD45 isoform that is analogous to murine B220 and is associated with altered O-glycan synthesis and onset of apoptosis." in: Cellular immunology, Vol. 213, Issue 1, pp. 72-81, (2001) (PubMed).

Driver, McHeyzer-Williams, Cool, Stetson, McHeyzer-Williams: "Development and maintenance of a B220- memory B cell compartment." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 3, pp. 1393-405, (2001) (PubMed).

Kobata, Takasaki, Asahara, Hong, Masuko-Hongo, Kato, Hirose, Shirai, Kayagaki, Yagita, Okumura, Nishioka: "Apoptosis with FasL+ cell infiltration in the periphery and thymus of corrected autoimmune mice." in: Immunology, Vol. 92, Issue 2, pp. 206-13, (1998) (PubMed).

Sagara, Sugaya, Tokoro, Tanaka, Takano, Kodama, Nakauchi, Takahama: "B220 expression by T lymphoid progenitor cells in mouse fetal liver." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 666-76, (1997) (PubMed).

Puzanov, Bennett, Kumar: "IL-15 can substitute for the marrow microenvironment in the differentiation of natural killer cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 10, pp. 4282-5, (1996) (PubMed).

Krop, de Fougerolles, Hardy, Allison, Schlissel, Fearon: "Self-renewal of B-1 lymphocytes is dependent on CD19." in: European journal of immunology, Vol. 26, Issue 1, pp. 238-42, (1996) (PubMed).

Renno, Hahne, Tschopp, MacDonald: "Peripheral T cells undergoing superantigen-induced apoptosis in vivo express B220 and upregulate Fas and Fas ligand." in: The Journal of experimental medicine, Vol. 183, Issue 2, pp. 431-7, (1996) (PubMed).

Rolink, ten Boekel, Melchers, Fearon, Krop, Andersson: "A subpopulation of B220+ cells in murine bone marrow does not express CD19 and contains natural killer cell progenitors." in: The Journal of experimental medicine, Vol. 183, Issue 1, pp. 187-94, (1996) (PubMed).

Domiati-Saad, Ogle, Justement: "Administration of anti-CD45 mAb specific for a B cell-restricted epitope abrogates the B cell response to a T-dependent antigen in vivo." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 11, pp. 5936-47, (1994) (PubMed).

George, Rath, Shroff, Wang, Durdik: "Ligation of CD45 on B cells can facilitate production of secondary Ig isotypes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 3, pp. 1014-21, (1994) (PubMed).

Laouar, Ezine: "In vivo CD4+ lymph node T cells from lpr mice generate CD4-CD8-B220+TCR-beta low cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 9, pp. 3948-55, (1994) (PubMed).

Ballas, Rasmussen: "Lymphokine-activated killer cells. VII. IL-4 induces an NK1.1+CD8 alpha+beta- TCR-alpha beta B220+ lymphokine-activated killer subset." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 1, pp. 17-30, (1993) (PubMed).

Allman, Ferguson, Cancro: "Peripheral B cell maturation. I. Immature peripheral B cells in adults are heat-stable antigenhi and exhibit unique signaling characteristics." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 8, pp. 2533-40, (1992) (PubMed).

Hathcock, Hirano, Murakami, Hodes: "CD45 expression by B cells. Expression of different CD45 isoforms by subpopulations of activated B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 7, pp. 2286-94, (1992) (PubMed).

Hardy, Carmack, Shinton, Kemp, Hayakawa: "Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow." in: The Journal of experimental medicine, Vol. 173, Issue 5, pp. 1213-25, (1991) (PubMed).

Asensi, Kimeno, Kawamura, Sakumoto, Nomoto: "Treatment of autoimmune MRL/lpr mice with anti-B220 monoclonal antibody reduces the level of anti-DNA antibodies and lymphadenopathies." in: Immunology, Vol. 68, Issue 2, pp. 204-8, (1989) (PubMed).

Coffman: "Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development." in: Immunological reviews, Vol. 69, pp. 5-23, (1983) (PubMed).

Target Details for CD45

Target: CD45 (PTPRC) (Protein tyrosine Phosphatase, Receptor Type, C (PTPRC))

Alternative Name: CD45R/B220

Background: The rat anti-mouse CD45R antibody (clone RA3-6B2) has been reported to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. It is expressed on B lymphocytes at all stages from pro-B through mature and activated B cell, but it is decreased on plasma cells and a subset of memory B cells. The levels of CD45R expression on the B-cell lineage appear to be developmentally regulated. It is also reportedly found on the abnormal T cells involved in the lymphadenopathy of lpr/lpr and gld/gld mutant mice, on lytically active subsets of lymphokine-activated killer cells (NK cells and non-MHC-restricted CTL), on apoptotic T lymphocytes of mice injected with bacterial superantigen, on a population of NK-cell precursors in the bone marrow, and on B-lymphocyte, T-lymphocyte, and macrophage progenitors in fetal liver. The CD45R antigen has been reported not to be on hematopoietic stem cells, naive T lymphocytes, or MHC-restricted CTL. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the mouse are cell type-, maturation, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction. CD45R is commonly used as a pan B-cell marker, however, CD19 expression, detectable by the rat anti-mouse CD19 antibody (clone 1D3), is reported to be more restricted to the B-cell lineage. The rat anti-mouse CD45R antibody (clone RA3-6B2) has been reported to enhance isotype switching during in vitro B-cell responses and to inhibit in vivo B-cell responses. Cross-reaction of the RA3-6B2 clone with activated human T lymphocytes has also been reportedly observed.
Synonyms: B220, Ly-5, CD45R, LCA, Ptprc, Protein tyrosine phosphatase receptor type C

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, CXCR4-mediated Signaling Events, BCR Signaling