TNFRSF18 antibody

Catalog No. ABIN1176970

This Rat Monoclonal antibody specifically detects TNFRSF18 in FACS and BR. It exhibits reactivity toward Mouse. It has been mentioned in 5+ publications

Quick Overview for TNFRSF18 antibody (ABIN1176970)

Target: TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TNFRSF18 antibody is un-conjugated

Application: Flow Cytometry (FACS), Blocking Reagent (BR)

Clone: DTA

Product Details anti-TNFRSF18 Antibody

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse CD25+ CD4+ T Cell Line

Isotype: IgG2b

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-TNFRSF18 antibody (ABIN1176970)

Ko, Yamazaki, Nakamura, Nishioka, Hirota, Yamaguchi, Shimizu, Nomura, Chiba, Sakaguchi: "Treatment of advanced tumors with agonistic anti-GITR mAb and its effects on tumor-infiltrating Foxp3+CD25+CD4+ regulatory T cells." in: The Journal of experimental medicine, Vol. 202, Issue 7, pp. 885-91, (2005) (PubMed).

Ji, Liao, Faubion, Abadía-Molina, Cozzo, Laroux, Caton, Terhorst: "Cutting edge: the natural ligand for glucocorticoid-induced TNF receptor-related protein abrogates regulatory T cell suppression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 10, pp. 5823-7, (2004) (PubMed).

Tone, Tone, Adams, Yates, Frewin, Cobbold, Waldmann: "Mouse glucocorticoid-induced tumor necrosis factor receptor ligand is costimulatory for T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 25, pp. 15059-64, (2003) (PubMed).

Shimizu, Yamazaki, Takahashi, Ishida, Sakaguchi: "Stimulation of CD25(+)CD4(+) regulatory T cells through GITR breaks immunological self-tolerance." in: Nature immunology, Vol. 3, Issue 2, pp. 135-42, (2002) (PubMed).

Nocentini, Giunchi, Ronchetti, Krausz, Bartoli, Moraca, Migliorati, Riccardi: "A new member of the tumor necrosis factor/nerve growth factor receptor family inhibits T cell receptor-induced apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 12, pp. 6216-21, (1997) (PubMed).

Target Details for TNFRSF18

Target: TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))

Alternative Name: GITR

Background: The DTA-1 antibody reacts with GITR [Glucocorticoid-induced Tumor necrosis factor (TNF) receptor family-Related], a 66-70-kDa homodimer glycoprotein that is a member of the TNF receptor superfamily and is also known as TNFRSF18. As its name implies, GITR expression was first detected in T lymphocytes that had been treated with dexamethasone, a glucocorticoid. In normal naive mice, GITR is expressed at moderate levels on CD25-positive/CD4-positive/CD8a-negative thymocytes and on CD25-positive/CD4-positive/CD45RB-low splenocytes. It is also expressed at low levels on splenic CD25-negative/CD4-positive/CD45RB-low T lymphocytes, B lymphocytes, macrophages, and dendritic cells. Activation of T and B lymphocytes upregulates GITR expression. GITR is a costimulatory receptor that plays an important role in Regulatory T (Treg)-cell functions, and a GITR Ligand has been detected on B lymphocytes, macrophages, and dendritic cells. mAb DTA-1 abrogates suppression by Treg cells without affecting their proliferative response, while it is co-stimulatory for T lymphocytes that are not Treg cells.

Pathways: Cancer Immune Checkpoints