ITGA5 antibody

Catalog No. ABIN1176992

Product Details anti-ITGA5 Antibody

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Blocking Reagent (BR), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Mouse mast cell line MC/9

Clone: 5H10-27

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-ITGA5 antibody (ABIN1176992)

Cukierman, Pankov, Stevens, Yamada: "Taking cell-matrix adhesions to the third dimension." in: Science (New York, N.Y.), Vol. 294, Issue 5547, pp. 1708-12, (2001) (PubMed).

Yang, Hynes: "Fibronectin receptor functions in embryonic cells deficient in alpha 5 beta 1 integrin can be replaced by alpha V integrins." in: Molecular biology of the cell, Vol. 7, Issue 11, pp. 1737-48, (1997) (PubMed).

Ruppert, Aigner, Hubbe, Yagita, Altevogt: "The L1 adhesion molecule is a cellular ligand for VLA-5." in: The Journal of cell biology, Vol. 131, Issue 6 Pt 2, pp. 1881-91, (1996) (PubMed).

Rich, Van Nood, Lee: "Role of alpha 5 beta 1 integrin in TGF-beta 1-costimulated CD8+ T cell growth and apoptosis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 7, pp. 2916-23, (1996) (PubMed).

Gomez, Cano: "Expression of beta 1 integrin receptors in transformed mouse epidermal keratinocytes: upregulation of alpha 5 beta 1 in spindle carcinoma cells." in: Molecular carcinogenesis, Vol. 12, Issue 3, pp. 153-65, (1995) (PubMed).

Hardy, Minguell: "Modulation of the adhesion of hemopoietic progenitor cells to the RGD site of fibronectin by interleukin 3." in: Journal of cellular physiology, Vol. 164, Issue 2, pp. 315-23, (1995) (PubMed).

Halvorson, Coligan: "Enhancement of VLA integrin receptor function on thymocytes by cAMP is dependent on the maturation stage of the thymocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 10, pp. 4567-74, (1995) (PubMed).

Kinashi, Springer: "Adhesion molecules in hematopoietic cells." in: Blood cells, Vol. 20, Issue 1, pp. 25-44, (1995) (PubMed).

Target Details for ITGA5

Target: ITGA5 (Integrin, alpha 5 (ITGA5))

Alternative Name: CD49e (ITGA5 Products)

Synonyms: ITGA5 antibody, Cd49e antibody, Fnra antibody, VLA5 antibody, CD49e antibody, FNRA antibody, VLA5A antibody, Itga5_retired antibody, integrin subunit alpha 5 antibody, integrin alpha 5 (fibronectin receptor alpha) antibody, ITGA5 antibody, Itga5 antibody

Background: The 5H10-27 (MFR5) antibody reacts with the alpha5 chain of the integrin alpha5beta1 fibronectin receptor (CD49e/CD29, VLA-5) on thymocytes, activated T lymphocytes, mast cells, and a variety of mouse cell lines, but not splenocytes. Soluble 5H10-27 (MFR5) antibody has been reported to inhibit VLA-5-mediated functions in vitro. In addition, immobilized mAb 5H10-27 (MFR5) has been demonstrated to costimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3e antibody.
Synonyms: Integrin alpha5 chain

Pathways: Integrin Complex