IL-4 antibody

Catalog No. ABIN1177017

Product Details anti-IL-4 Antibody

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Neutralization (Neut)

Brand: BD Pharmingen™

Characteristics: Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-4. Neutralization activity may be measured with a proliferation assay using 1 ng/mL recombinant mouse IL-4 (Cat. No. 550067) to stimulate CTLL-2 cells at 2x10^4 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-4 for 60 minutes).
50% Neutralization (ND50) at 1 - 50 ng/mL
>95% Neutralization at 50 - 300 ng/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Partially Purified Mouse IL-4

Clone: 11B11

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL-4 antibody (ABIN1177017)

Openshaw, Murphy, Hosken, Maino, Davis, Murphy, OGarra: "Heterogeneity of intracellular cytokine synthesis at the single-cell level in polarized T helper 1 and T helper 2 populations." in: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1357-67, (1995) (PubMed).

Lindqvist, Lundström, Oker-Blom, Akerman: "Enhanced IL-4-mediated D10.G4.1 proliferation with suboptimal concentrations of anti-IL-4 receptor monoclonal antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 2, pp. 394-8, (1993) (PubMed).

Haak-Frendscho, Brown, Iizawa, Wagner, Czuprynski: "Administration of anti-IL-4 monoclonal antibody 11B11 increases the resistance of mice to Listeria monocytogenes infection." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 12, pp. 3978-85, (1992) (PubMed).

Swain, Weinberg, English, Huston: "IL-4 directs the development of Th2-like helper effectors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 145, Issue 11, pp. 3796-806, (1991) (PubMed).

Ohara, Paul: "Production of a monoclonal antibody to and molecular characterization of B-cell stimulatory factor-1." in: Nature, Vol. 315, Issue 6017, pp. 333-6, (1985) (PubMed).

Target Details for IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Alternative Name: IL-4 (IL4 Products)

Synonyms: IL4 antibody, IL-4 antibody, BCGF-1 antibody, BCGF1 antibody, BSF-1 antibody, BSF1 antibody, Il-4 antibody, Il4e12 antibody, IL2 antibody, interleukin 4 antibody, IL4 antibody, Il4 antibody

Background: The 11B11 antibody reacts with mouse interleukin-4 (IL-4). The immunogen used to generate the 11B11 hybridoma was partially purified mouse IL-4 from PMA-stimulated EL-4 supernatant. The purified or unconjugated form of this antibody has been reported to be neutralizing.

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation