IRF7 antibody (pSer477) (Alexa Fluor 488)

Catalog No. ABIN1177072

This Mouse Monoclonal antibody specifically detects IRF7 in ICS. It exhibits reactivity toward Human. It has been mentioned in 3+ publications

Quick Overview for IRF7 antibody (pSer477) (Alexa Fluor 488) (ABIN1177072)

Target: IRF7 (Interferon Regulatory Factor 7 (IRF7))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IRF7 antibody is conjugated to Alexa Fluor 488

Application: Intracellular Staining (ICS)

Clone: K47-671

Product Details anti-IRF7 Antibody (Alexa Fluor 488)

Binding Specificity: pSer477

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human IRF-7 Peptide

Isotype: IgG1 kappa

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

References for anti-IRF7 antibody (Alexa Fluor 488) (ABIN1177072)

Paz, Sun, Nakhaei, Romieu-Mourez, Goubau, Julkunen, Lin, Hiscott: "Induction of IRF-3 and IRF-7 phosphorylation following activation of the RIG-I pathway." in: Cellular and molecular biology (Noisy-le-Grand, France), Vol. 52, Issue 1, pp. 17-28, (2006) (PubMed).

Hoshino, Sugiyama, Matsumoto, Tanaka, Saito, Hemmi, Ohara, Akira, Kaisho: "IkappaB kinase-alpha is critical for interferon-alpha production induced by Toll-like receptors 7 and 9." in: Nature, Vol. 440, Issue 7086, pp. 949-53, (2006) (PubMed).

Sharma, tenOever, Grandvaux, Zhou, Lin, Hiscott: "Triggering the interferon antiviral response through an IKK-related pathway." in: Science (New York, N.Y.), Vol. 300, Issue 5622, pp. 1148-51, (2003) (PubMed).

Target Details for IRF7

Target: IRF7 (Interferon Regulatory Factor 7 (IRF7))

Alternative Name: IRF-7

Background: Interferon regulatory factor 7 (IRF-7) is a transcription factor that regulates anti-viral defenses by controlling the induction of type-I interferon (IFN) responses. IRF-7 expression is induced in lymphoid cells by virus infection, as well as by IFN, lipopolysaccharide, and TNF-a. IRF-7 responses are initiated by Toll-like receptors (TLR) or the cytoplasmic protein retinoic acid inducible gene I (RIG-I). Upon TLR activation, it forms cytoplasmic complexes with MyD88, an adaptor in the TLR signaling pathways. The TLR-dependent and RIG-I-dependent pathways activate kinases, such as IKK-e and TBK1, that phosphorylate IRF-7 and induce movement of IRF-7-containing complexes to the nucleus, where it preferentially activates IFN-a promoters. The K47-671 monoclonal antibody recognizes human IRF-7 phosphorylated at serines 477 and 479 (pS477/pS479). Our in-house testing is performed on a cell line that has been co-transfected with TBK1 and IRF-7. Phosphorylation of IRF-7 in the transfectants requires virus infection or over-expression of a signaling molecule of the RIG-I pathway, such as TBK1. Phosphorylation of endogenous IRF-7 in untransfected cells has not yet been detected. We confirmed that mAb K47-671 does not cross-react with TBK1 by Western blot analysis using the purified antibody.

Pathways: TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Autophagy