IRS1 antibody (pTyr896) (Alexa Fluor 647)

Catalog No. ABIN1177074

Product Details anti-IRS1 Antibody (Alexa Fluor 647)

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Binding Specificity: pTyr896

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This IRS1 antibody is conjugated to Alexa Fluor 647

Application: Intracellular Staining (ICS)

Brand: BD Phosflow™

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography

Immunogen: Phosphorylated Human IRS-1 Peptide

Clone: K9-211

Isotype: IgG2a kappa

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-IRS1 antibody (Alexa Fluor 647) (ABIN1177074)

Gual, Le Marchand-Brustel, Tanti: "Positive and negative regulation of insulin signaling through IRS-1 phosphorylation." in: Biochimie, Vol. 87, Issue 1, pp. 99-109, (2005) (PubMed).

White: "IRS proteins and the common path to diabetes." in: American journal of physiology. Endocrinology and metabolism, Vol. 283, Issue 3, pp. E413-22, (2002) (PubMed).

Burks, White: "IRS proteins and beta-cell function." in: Diabetes, Vol. 50 Suppl 1, pp. S140-5, (2001) (PubMed).

Paz, Liu, Shorer, Hemi, LeRoith, Quan, Kanety, Seger, Zick: "Phosphorylation of insulin receptor substrate-1 (IRS-1) by protein kinase B positively regulates IRS-1 function." in: The Journal of biological chemistry, Vol. 274, Issue 40, pp. 28816-22, (1999) (PubMed).

Target Details for IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Alternative Name: IRS-1 (IRS1 Products)

Synonyms: HIRS-1 antibody, G972R antibody, IRS-1 antibody, IRS1IRM antibody, irs1 antibody, irsu antibody, IRS1 antibody, irs-1 antibody, irs1-a antibody, insulin receptor substrate 1 antibody, insulin receptor substrate 2 antibody, insulin receptor substrate 1 S homeolog antibody, IRS1 antibody, Irs1 antibody, irs2 antibody, irs1.S antibody

Background: The IRS (Insulin Receptor Substrate) proteins IRS-1, IRS-2, IRS-3, and IRS-4 are major substrates of the insulin receptor and the insulin-like growth factor-1 (IGF-1) receptor tyrosine kinases. IRS proteins contain an N-terminal pleckstrin homology (PH) domain, a phosphotyrosine-binding (PTB) domain, and multiple tyrosine phosphorylation sites in the C-terminus. The IRS-1 protein is widely expressed and, along with IRS-2, mediates somatic growth and carbohydrate metabolic responses to insulin. Following insulin receptor ligation, IRS-1 binds to the juxtamembrane region of the receptor via the PH and PTB domains and is tyrosine phosphorylated, which facilitates its interaction with SH2 domain-containing signaling proteins. Specifically, the phosphorylated tyrosine 896 (pY896) of human IRS-1 is a major binding site for the GRB2 (Growth-factor Receptor-Bound protein 2) adaptor protein. After IRS-1 activation, negative and positive feedback regulates dephosphorylation of its tyrosine sites, which ultimately regulates the magnitude and/or duration of the downstream pleiotropic responses to insulin and IGF-1. The K9-211 monoclonal antibody recognizes pY896 of human IRS-1. The orthologous phosphorylation sites of mouse and rat IRS-1 are Y891 and Y895, respectively.

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process