Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Phospholipase C gamma 2 antibody (pTyr759) (PE)

PLCG2 Reactivity: Human, Rat, Mouse ICS Host: Mouse Monoclonal K86-689-37 PE
Catalog No. ABIN1177149
  • Target See all Phospholipase C gamma 2 (PLCG2) Antibodies
    Phospholipase C gamma 2 (PLCG2)
    Binding Specificity
    • 31
    • 22
    • 16
    • 16
    • 7
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr759
    Reactivity
    • 131
    • 67
    • 53
    • 6
    • 4
    • 4
    • 4
    • 4
    • 3
    • 1
    Human, Rat, Mouse
    Host
    • 125
    • 6
    Mouse
    Clonality
    • 121
    • 10
    Monoclonal
    Conjugate
    • 60
    • 7
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    This Phospholipase C gamma 2 antibody is conjugated to PE
    Application
    • 96
    • 40
    • 40
    • 36
    • 35
    • 23
    • 19
    • 18
    • 11
    • 7
    • 5
    • 2
    • 2
    • 1
    Intracellular Staining (ICS)
    Brand
    BD Phosflow™
    Purification
    Purified from tissue culture supernatant or ascites by affinity chromatography
    Immunogen
    Phosphorylated Human PLC-gamma2 Peptide
    Clone
    K86-689-37
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product PLCG2 Primary Antibody
  • Sample Volume
    20 μL
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
  • Kim, Sekiya, Poulin, Bae, Rhee: "Mechanism of B-cell receptor-induced phosphorylation and activation of phospholipase C-gamma2." in: Molecular and cellular biology, Vol. 24, Issue 22, pp. 9986-99, (2004) (PubMed).

  • Target
    Phospholipase C gamma 2 (PLCG2)
    Alternative Name
    PLC-gamma 2 (PLCG2 Products)
    Synonyms
    PLCgamma2 antibody, Plcg-2 antibody, APLAID antibody, FCAS3 antibody, phospholipase C, gamma 2 antibody, phospholipase C gamma 2 antibody, phospholipase C, gamma 2 (phosphatidylinositol-specific) antibody, Plcg2 antibody, PLCG2 antibody
    Background
    The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLC-gamma is the only member that contains SH2 and SH3 domains. These domains enable it to interact with protein tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: PLC-g1, which is ubiquitously expressed, and PLC-g2, which is found primarily in the hematopoietic system. PLC-g2-null mice exhibit impaired B lymphocyte maturation and defects in Fc receptor functions. Phosphorylation of PLC-g2 at tyrosines 753 and 759 (Y759) is required for activation of PLC-g2 enzyme activity. PLC-g2 phosphorylation at Y759 can be induced by stimulation of the B cell receptor in Ramos cells, the collagen receptor in platelets, and the T cell receptor in Jurkat cells, and it occurs downstream of Btk and BLNK in the signaling cascade of activated B cells. The K86-689.37.73 antibody recognizes PLC-g2 phosphorylated at Y759 in the SH2-SH3 linker region.
    Pathways
    RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling, BCR Signaling
You are here:
Support