STAT4 antibody (pTyr693) (Alexa Fluor 488)

Catalog No. ABIN1177206

The Alexa Fluor 488-conjugated Mouse Monoclonal anti-STAT4 antibody (Clone 38-p) (ABIN1177206) specifically detects STAT4 in ICS. The antibody is reactive with Human and Mouse samples.

Quick Overview for STAT4 antibody (pTyr693) (Alexa Fluor 488) (ABIN1177206)

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This STAT4 antibody is conjugated to Alexa Fluor 488

Application: Intracellular Staining (ICS)

Clone: 38-p

Product Details anti-STAT4 Antibody (Alexa Fluor 488)

Binding Specificity: pTyr693

Brand: BD Phosflow™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human Stat4 (pY693)

Isotype: IgG2b kappa

Application Details

Sample Volume: 20 μL

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

Target Details for STAT4

Target: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Alternative Name: Stat4

Background: The Stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Seven mammalian Stat proteins have been identified: Stat1-4, Stat5a, 5b, and Stat6. Stat4 has been shown to play an important role in development of T helper cells, specifically the Th1 subset. Stat4 is activated by IL-12 and by type 1 interferons. Knockout mice supported the role that Stat4 plays in IL-12 signaling because lymphocytes from Stat 4-/- mice could not differentiate into Th1 cells or produce IFNgamma in response to treatment with IL-12. IFNgamma plays an important role in host defense. A key component in the activation of Stat4 is the phosphorylation on tyrosine and serine residues in response to IL-12 stimulation. IL-12 stimulation leads to the phosphorylation of Stat4 on tyrosine 693 and serine 721. Transcriptional activity of Stat4 has been shown to be significantly reduced when residues Y693 and S721 are mutated. This antibody is routinely tested by flow cytometric analysis.

Pathways: JAK-STAT Signaling