IL-10 antibody

Catalog No. ABIN1177280

Product Details anti-IL-10 Antibody

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Reactivity: Human, Virus

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-10 antibody is un-conjugated

Application: ELISA, Neutralization (Neut), Intracellular Staining (ICS)

Brand: BD Pharmingen™

Characteristics: Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human IL-10. Neutralization activity may be measured with a proliferation assay using 3 ng/mL recombinant human IL-10 (Cat.No. 554611), co-stimulated with 0.1 ng/mL recombinant mouse IL-4 (Cat.No. 550067), to stimulate MC/9 or D36 cells as indicator cells (i.e preincubation of the antibody with recombinant human IL-10 for 60 min)
50% Neutralization (ND50) at 0.2 - 4.0 ng/mL
>95% Neutralization at 3 - 30 ng/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Recombinant Human IL-10

Clone: JES3-9D7

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL-10 antibody (ABIN1177280)

Abrams: "Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies." in: Current protocols in immunology / edited by John E. Coligan ... [et al.], Vol. Chapter 6, pp. Unit 6.20, (2008) (PubMed).

Burdin, Péronne, Banchereau, Rousset: "Epstein-Barr virus transformation induces B lymphocytes to produce human interleukin 10." in: The Journal of experimental medicine, Vol. 177, Issue 2, pp. 295-304, (1993) (PubMed).

Abrams, Roncarolo, Yssel, Andersson, Gleich, Silver: "Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples." in: Immunological reviews, Vol. 127, Issue 9-10, pp. 5-24, (1992) (PubMed).

Gotlieb, Abrams, Watson, Velu, Berek, Martínez-Maza: "Presence of interleukin 10 (IL-10) in the ascites of patients with ovarian and other intra-abdominal cancers." in: Cytokine, Vol. 4, Issue 5, pp. 385-90, (1992) (PubMed).

Target Details for IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL-10 (IL10 Products)

Synonyms: CSIF antibody, GVHDS antibody, IL-10 antibody, IL10A antibody, TGIF antibody, Il-10 antibody, IL10X antibody, interleukin 10 antibody, IL10 antibody, Il10 antibody

Background: The JES3-9D7 antibody reacts with human IL-10 and viral IL-10. The immunogen used to generate the JES3-9D7 hybridoma was recombinant human IL-10 expressed in COS cells.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints