IL-2 antibody

Catalog No. ABIN1177301

The Rat Monoclonal anti-IL-2 antibody has been validated for WB and Neut. It is suitable to detect IL-2 in samples from Mouse. There are 3+ publications available.

Quick Overview for IL-2 antibody (ABIN1177301)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: Western Blotting (WB), Neutralization (Neut)

Clone: S4B6

Product Details anti-IL-2 Antibody

Brand: BD Pharmingen™

Characteristics: Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant mouse IL-2. Neutralization activity may be measured with a proliferation assay using 80 pg/mL recombinant mouse IL-2 (Cat. No. 550069) to stimulate CTLL-2 cells at 1x10^5 cells/mL as indicator cells (i.e. preincubation of the antibody with recombinant mouse IL-2 for 60 minutes).
50% Neutralization (ND50) at 5 - 30 ng/mL
>95% Neutralization at 30 - 80 ng/mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: T cell clone supernatant

Isotype: IgG2a

Application Details

Application Notes: Western Blot: The S4B6 antibody (purified format) has been reported to be useful for Western blotting. A concentration of 1-5 µg/mL (in conjunction with an AKP-conjugated goat anti-rat Ig secondary antibody) has been reported to enable detection of ≤ 100 ng/lane of recombinant mouse IL-2 (Cat. No. 550069) under reducing conditions.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing protein stabilizer, no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-IL-2 antibody (ABIN1177301)

Svetić, Jian, Lu, Finkelman, Gause: "Brucella abortus induces a novel cytokine gene expression pattern characterized by elevated IL-10 and IFN-gamma in CD4+ T cells." in: International immunology, Vol. 5, Issue 8, pp. 877-83, (1993) (PubMed).

Kubo, Cinader: "Polymorphism of age-related changes in interleukin (IL) production: differential changes of T helper subpopulations, synthesizing IL 2, IL 3 and IL 4." in: European journal of immunology, Vol. 20, Issue 6, pp. 1289-96, (1990) (PubMed).

Gillis, Ferm, Ou, Smith: "T cell growth factor: parameters of production and a quantitative microassay for activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 120, Issue 6, pp. 2027-32, (1978) (PubMed).

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2

Background: The S4B6 antibody reacts with mouse interleukin-2 (IL-2). The immunogen used to generate the S4B6 hyridoma was a T cell clone supernatant. This is a neutralizing antibody.

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation