ITGB1 antibody

Catalog No. ABIN1177328

Product Details anti-ITGB1 Antibody

Target: ITGB1 (Integrin beta 1 (ITGB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGB1 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Clone: HUTS-21

Isotype: IgG2a kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-ITGB1 antibody (ABIN1177328)

García-Gila, Cabañas, García-Pardo: "Analysis of the activation state of alpha4beta1 integrin in human B cell lines derived from myeloma, leukemia or lymphoma." in: FEBS letters, Vol. 418, Issue 3, pp. 337-40, (1998) (PubMed).

Target Details for ITGB1

Target: ITGB1 (Integrin beta 1 (ITGB1))

Alternative Name: CD29 (ITGB1 Products)

Background: Reacts with the 130 kDa integrin beta1 subunit, CD29, that is expressed following cell activation as a heterodimeric complex with one of nine distinct alpha subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. The HUTS-21 beta1 epitope is weakly expressed on resting lymphocytes, but is upregulated by TPA, Ca++ ionophore, or another CD29 antibody, TS2/16. This upregulation is Mn++ dependent and not supported by other divalent cations such as Ca++ and/or Mg++. The VLA receptors have a broad cellular distribution and interact with ligands like VCAm-1 and MAdCAM-1 during cell adhesion. They also interact with extra-cellular matrices including collagen, laminin, fibronectin and others. HUTS-21 antibody enhances adherence of TPA-activated lymphocytes to fibronectin.
Synonyms: Integrin beta1 Chain

Pathways: Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor, Integrin Complex, SARS-CoV-2 Protein Interactome