POLM antibody (AA 6-18)

Catalog No. ABIN117919

This Rabbit Polyclonal antibody specifically detects POLM in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for POLM antibody (AA 6-18) (ABIN117919)

Target: POLM (Polymerase (DNA Directed), mu (POLM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLM antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-POLM Antibody

Binding Specificity: AA 6-18

Sequence: Peptide sequence: R-R-A-R-V-G-S-P-S-G-D-A-A-C

Specificity: This is an affinity purified antibody produced by Immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Purification: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 6-18 of Human POLM µ (DNA polymerase mu). POLM µ is a template-directed terminal deoxynucleotidetransferase that shows a preferential expression in proliferating lymphoid cells.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA against the immunizingpeptide. Reactivity in other immunoassays is unknown. This product has been assayed byELISA against 0.1 μg of the immunizing peptide. A 1: 33,000 to 1: 135,000 dilution of the antibody is recommended for this assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 2.1 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for POLM

Target: POLM (Polymerase (DNA Directed), mu (POLM))

Alternative Name: DNA Polymerase mu

Background: DNA ploymerase mu is a template directed terminal deoxynucleotidetransferase that shows a preferential expression in proliferating lymphoid cells. It seems to act as an Ig mutase which is responsible for immunoglobulin (Ig) gene hypermutation.Synonyms: POLM, Pol Mu, Tdt-N, TdtN

Gene ID: 27434

NCBI Accession: NP_037416

UniProt: Q9NP87

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response