ANAPC11 antibody (C-Term)

Catalog No. ABIN117941

The Rabbit Polyclonal anti-ANAPC11 antibody is suitable to detect ANAPC11 in samples from Human. It has been validated for WB, EIA and IP.

Quick Overview for ANAPC11 antibody (C-Term) (ABIN117941)

Target: ANAPC11 (Anaphase Promoting Complex Subunit 11 (ANAPC11))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANAPC11 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunoprecipitation (IP)

Product Details anti-ANAPC11 Antibody

Binding Specificity: AA 76-84, C-Term

Sequence: Human APC11 (84 aa 9.8 kDa): 1 mkvkikcwng vatwlwvand encgicrmaf ngccpdckvp gddcplvwgq cshcfhmhci 61 lkwlhaqqvq qhcpmcrqew kfke

Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This antibody reacts with human and mouse APC11. Cross reactivity may also occur with APC11 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and ROC2.

Purification: Delipidation and defibrination.

Immunogen: This APC11 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 76-84 of Human APC11 (C-terminal) coupled to KLH.

Application Details

Application Notes: Western blot (1: 500-1: 1,000). ELISA (1: 2,000-1: 10,000). Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein andprotein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins (APC2) does occur. A 9.8 kDa bandcorresponding to human APC11 is detected. Most cell lines or tissues expressing APC11 canbe used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 85 mg/mL (by Refractometry)

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -80 °C for longer.

Target Details for ANAPC11

Target: ANAPC11 (Anaphase Promoting Complex Subunit 11 (ANAPC11))

Alternative Name: APC11 / ANAPC11

Background: APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization. APC11 is expressed at high levels in skeletal muscle and heart, in moderate levels in brain, kidney, and liver, and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated.Synonyms: Anaphase-promoting complex subunit 11, Cyclosome subunit 11, HSPC214, Hepatocellular carcinoma-associated RING finger protein

Gene ID: 51529

NCBI Accession: NP_001002244

UniProt: Q9NYG5