Glycogen Synthase antibody (pSer641)

Catalog No. ABIN117965

The Rabbit Polyclonal anti-Glycogen Synthase antibody has been validated for WB and EIA. It is suitable to detect Glycogen Synthase in samples from Human and Mouse.

Quick Overview for Glycogen Synthase antibody (pSer641) (ABIN117965)

Target: Glycogen Synthase (GYS)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Glycogen Synthase antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Glycogen Synthase Antibody

Binding Specificity: pSer641

Sequence: A-Q-G-Y-R-Y-P-R-P-A-(pS)-V-P (aa 631-642)

Specificity: This affinity purified antibody is directed against human muscle glycogen synthase. The product was affinity purified from monospecific antiserum by Immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS640 of human muscle glycogen synthase. Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1 % by ELISA. Cross reactivity with muscle glycogen synthase occurs in mouse tissue. Reactivity with muscle glycogen synthase from other sources has not been determined.

Purification: Immunoaffinity chromatography.

Immunogen: Human Muscle Glycogen Synthase phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Application Details

Application Notes: This phospho specific polyclonal antibody was tested by Immunoblotting and ELISA. By ELISA the antibody was found to be reactive with the phosphorylated form of theimmunizing peptide and minimally reactive with the non-phosphorylated form of theimmunizing peptide. Immunoblotting will detect human and mouse muscle glycogensynthase. Although not tested, this antibody is likely functional in Immunohistochemistryand Immunoprecipitation. This product has been assayed against 0.1 g of phosphorylatedpeptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as asubstrate for 30 minutes at room temperature. A working dilution of 1: 30,000 to 1: 150,000is suggested for this product. Less than 0.2 % cross-reactivity was detected against thenon-phosphorylated form o f the immunizing peptide. Dilute the antibody 1: 1,000 for

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C one month or (in aliquots) at -20 °C for longer.

Target Details for Glycogen Synthase

Target: Glycogen Synthase (GYS)

Alternative Name: Glycogen Synthase

Background: Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3β that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S640. Phosphorylation of GS at S640 has been associated with Antiphospholipid Antibody Syndrome.Synonyms: GYS, GYS1, muscle Glycogen Synthase

Gene ID: 2997

NCBI Accession: NP_002094

UniProt: P13807