Biotin antibody (Texas Red (TR))

Catalog No. ABIN118072

This Goat Polyclonal antibody specifically detects Biotin in WB, FACS, IHC (p), IP, EIA and IHC (fro).

Quick Overview for Biotin antibody (Texas Red (TR)) (ABIN118072)

Target: Biotin

Host: Goat

Clonality: Polyclonal

Conjugate: This Biotin antibody is conjugated to Texas Red (TR)

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Biotin Antibody (Texas Red (TR))

Characteristics: Absorption / Emission: 596 nm / 620 nm
Molar Ratio: 4.8 Moles Texas RedTM per mole of Goat IgG F(ab')2.

Purification: Immunoaffinity chromatography

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Application Details

Application Notes: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as otherantibody based fluorescent assays requiring lot-to-lot consistency. Flow cytometry: 1.2,000 - 1: 10,000. IF microscopy: 1: 500 - 1: 2,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water (or equivalent).

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. Restore with 0.5 ml of deionized water (or equivalent). For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for Biotin

Target: Biotin

Target Type: Chemical

Background: Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.