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CD4 antibody

This Rat Monoclonal antibody specifically detects CD4 in FACS. It exhibits reactivity toward Mouse.
Catalog No. ABIN118114

Quick Overview for CD4 antibody (ABIN118114)

Target

See all CD4 Antibodies
CD4

Reactivity

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Mouse

Host

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Rat

Clonality

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Monoclonal

Conjugate

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This CD4 antibody is un-conjugated

Application

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Flow Cytometry (FACS)

Clone

YTS191-1
  • Specificity

    This antibody recognises the murine CD4 cell surface antigen, expressed by a subset of T lymphocytes.

    Purification

    Protein G affinity chromatography

    Isotype

    IgG2b
  • Application Notes

    Flow cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cellsin 100 μL. Immunohistochemistry on frozen sections (3).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    Restrictions

    For Research Use only
  • Concentration

    1.0 mg/mL

    Buffer

    PBS, pH 7.4, containing 0.09 % sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target

    CD4

    Alternative Name

    CD4

    Background

    CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

    Gene ID

    12504

    NCBI Accession

    NP_038516

    UniProt

    P06332

    Pathways

    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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