IL-2 antibody

Catalog No. ABIN118387

This Rat Monoclonal antibody specifically detects IL-2 in IP and EIA. It exhibits reactivity toward Mouse.

Quick Overview for IL-2 antibody (ABIN118387)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: JES6-5H4

Product Details anti-IL-2 Antibody

Specificity: This antibody is specific for Interleukin-2 (IL-2).

Purification: Affinity chromatography on Protein G

Immunogen: Recombinant Mouse IL-2. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3.X63 Ag8.653 myeloma cell line.

Isotype: IgG2b

Application Details

Application Notes: ELISA. Immunoprecipitation. Flow cytometry: 1/10 - 1/50, Use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL, Membrane permeabilisation is required.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: Interleukin-2 / IL2

Background: IL-2 is a potent lymphoid cell growth factor which primarily acts on T lymphocytes.Synonyms: IL-2, T-cell growth factor, TCGF

Gene ID: 16183

NCBI Accession: NP_032392

UniProt: P04351

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation