CD4 antibody

Catalog No. ABIN118486

This Mouse Monoclonal antibody specifically detects CD4 in FACS, IHC (fro) and Func. It exhibits reactivity toward Human.

Quick Overview for CD4 antibody (ABIN118486)

Target: CD4

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: RPA-T4

Product Details anti-CD4 Antibody

Specificity: This antibody recognises CD4. Epitope mapping studies have shown that clone RPA-T4 recognises an epitope within domain 1 of the extracellular region of the CD4 Molecule. Clone RPA-T4 has been reported to block gp120-CD4 interaction and inhibit syncytium formation (See Voehringer, D. et al. for details).

Purification: Affinity Chromatography on Protein G

Endotoxin Level: Low Endotoxin

Immunogen: Human PHA blasts. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: Functional Assays. Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Immunohistochemistry on frozen sections: 1/200 - 1/2000. Recommended Positive Control: Human lymphatic tissue
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, None, None

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Upon receipt, store undiluted (in aliquots) at -20 °C.

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: CD4 is a 55kD cell surface glycoprotein that is expressed primarily on a subpopulation of T lymphocytes and on peripheral blood monocytes and tissue macrophages.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Gene ID: 920

NCBI Accession: NP_000607

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events