CD14 antibody

Catalog No. ABIN118508

The Mouse Monoclonal anti-CD14 antibody has been validated for FACS, WB, Func and EIA. It is suitable to detect CD14 in samples from Human, Monkey, Pig, Cow, Sheep, Dog, Rabbit, Goat, Cat and Mink.

Quick Overview for CD14 antibody (ABIN118508)

Target: CD14

Reactivity: Human, Monkey, Pig, Cow, Sheep, Dog, Rabbit, Goat, Cat, Mink

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Functional Studies (Func), Enzyme Immunoassay (EIA)

Clone: Tük4

Product Details anti-CD14 Antibody

Purification: Affinity chromatography on Protein G

Endotoxin Level: Low Endotoxin

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: 1/100 - 1/200, use 10 μL of suggested working dilution to label 1x10^6 cells or 100 μL whole blood. ELISA. Western Blot: under reducing conditions. Functional Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01 EU/ug

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

Gene ID: 9606

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades