VCAM1 antibody

Catalog No. ABIN118837

This Mouse Monoclonal antibody specifically detects VCAM1 in FACS, IP, IHC (fro) and EIA. It exhibits reactivity toward Human, Monkey and Pig.

Quick Overview for VCAM1 antibody (ABIN118837)

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reactivity: Human, Monkey, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This VCAM1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 1-G11B1

Product Details anti-VCAM1 Antibody

Specificity: This antibody recognises Human VCAM-1, a 110 kDa molecule whose ligand is VLA-4. The antigen is expressed on activated endothelial cells and some tissue macrophages, bone marrow fibroblasts and myoblasts. Cross reactivity to Porcine CD106 has been reported.

Purification: Affinity Chromatography on Protein A.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen tissues. ELISA. Immunoprecipitation. Flow cytometry (1/10 - 1/50). This antibody inhibits cellular adhesion mediated by VCAM-1. Removal of sodium azide is recommended prior to use in fuctional assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative and 0.1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for VCAM1

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Alternative Name: CD106 / VCAM1

Background: CD106 (known as VCAM1) is important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the integrins alpha4 beta1 (beta 1 integrin VLA4) and alpha4 beta7 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. VCAM1 is also expressed by several non endothelial cell types including some macrophages, follicular dendritic cells and bone marrow, stromal cells.Synonyms: INCAM-100, L1CAM, V-CAM 1, VCAM-1, Vascular cell adhesion protein 1

Gene ID: 7412

NCBI Accession: NP_001069

UniProt: P19320

Pathways: Carbohydrate Homeostasis